| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172494 |
| Chromosome: | chromosome 2 |
| Location: | 3358274 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095097 | FKB21,FKB17-2,FKB17B | (1 of 2) PTHR10516:SF290 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE; peptidyl-prolyl cis-trans isomerase, FKBP-type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGGCCGTTCATGCCCGGCCGTCTCAC |
| Internal bar code: | CTCTCCCGCTCTAGAGGCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.8304 |
| LEAP-Seq n confirming: | 2354 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAGTAAGGGTGTGTGGG |
| Suggested primer 2: | ATGGAAAGGCAAACTTGACG |