Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.172509 |
Chromosome: | chromosome_13 |
Location: | 3367308 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g586750 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAAAACCGGGACTCCAGTATTGGGTTTTG |
Internal bar code: | GAAAGGTATTGGGGACTGGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 672 |
LEAP-Seq percent confirming: | 98.2558 |
LEAP-Seq n confirming: | 169 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTACAGCAGTCCTGGCAACA |
Suggested primer 2: | GTGAGTGGATCTGTGTGCGT |