| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172511 |
| Chromosome: | chromosome 16 |
| Location: | 5943474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677000 | HSP70E,GEX27 | (1 of 1) K09489 - heat shock 70kDa protein 4 (HSPA4); Heat shock protein 70E | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACGTGCAAGGAATTGGTCAAGTTATCA |
| Internal bar code: | ATTGCTAGCGTACTGTCAGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 72 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGTACGACAGGCGTGGT |
| Suggested primer 2: | CTGTCTTGTGCTGCGAGTCT |