Insertion junction: LMJ.RY0402.172526_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.172526
Chromosome:chromosome 9
Location:7261214
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g412600 CYG52 (1 of 1) PF00072//PF00211 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc); Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATGATTGATTCACACAAGGAACAGGAC
Internal bar code:CGGCGCATCGGGTACATGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:214
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:630
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GACACATTCCACACGCATTC
Suggested primer 2:GACACCTGTGAGTGGGTGTG