| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172540 |
| Chromosome: | chromosome 4 |
| Location: | 1493998 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213904 | Mitogen-Activated Protein Kinase 3; (1 of 1) K02208 - cyclin-dependent kinase 8/11 [EC:2.7.11.22 2.7.11.23] (CDK8_11) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGTGTGGGTGGGGTTACATTCATGAT |
| Internal bar code: | CCCGCACGGGTTCTGACAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 32.1095 |
| LEAP-Seq n confirming: | 481 |
| LEAP-Seq n nonconfirming: | 1017 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAGGTCCCACTCCCAAT |
| Suggested primer 2: | CCAGGTGTGTGTGTGTGTGA |