| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172562 |
| Chromosome: | chromosome 2 |
| Location: | 5021932 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104350 | PDI5 | (1 of 1) PTHR10984//PTHR10984:SF37 - ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN // CELL REDOX HOMEOSTASIS PROTEIN-RELATED; Protein disulfide isomerase 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCTGCTGTTGGTTTTGCCATAGCCGAC |
| Internal bar code: | AGTGGCGGCCTTCTGCGTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 857 |
| LEAP-Seq percent confirming: | 99.585 |
| LEAP-Seq n confirming: | 8639 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAGGTCGTAGGTGAAGC |
| Suggested primer 2: | GACACATGACGCATAAACCG |