Insertion junction: LMJ.RY0402.172579_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.172579
Chromosome:chromosome 2
Location:1369544
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g083180 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACGAGGGTGTGGGCGCGGACACGACCG
Internal bar code:ACCGCAATGCTCTACCATACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:889
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGACTGCAGCTGATTGAA
Suggested primer 2:GCTTGTGATCTTTTGACGCA