| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172580 |
| Chromosome: | chromosome 16 |
| Location: | 2208261 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658650 | MYO1 | (1 of 1) PTHR13140//PTHR13140:SF256//PTHR13140:SF304 - MYOSIN // MYOSIN-14-RELATED // MYOSIN IA HEAVY CHAIN-RELATED; Myosin heavy chain, class XI | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAAGTCATCCTCCACCTTGATGACCTC |
| Internal bar code: | AGGACGGGTGTTGGGCGAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 99.4587 |
| LEAP-Seq n confirming: | 735 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCTCCAACACCTGAGC |
| Suggested primer 2: | CGACTAAGCGGAGTAGGCAC |