| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172598 |
| Chromosome: | chromosome 12 |
| Location: | 4074394 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517700 | NYC1,SDR21 | (1 of 2) K13606 - chlorophyll(ide) b reductase (NOL, NYC1); Chlorophyll b reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACAACATGCGCGCAGCGAAGGCGTAGG |
| Internal bar code: | TTCGGATATGCTATAGTTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 90.3074 |
| LEAP-Seq n confirming: | 2879 |
| LEAP-Seq n nonconfirming: | 309 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGGTGAGTACAAAGCAA |
| Suggested primer 2: | GATCGTGGGCTTGTTACGTT |