| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172607 |
| Chromosome: | chromosome_11 |
| Location: | 2737807 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g477100 | FAP97 | Flagellar Associated Protein, serine endopeptidase | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGAGGAGGAGGCGGGGGCCTCTTTGGCGC |
| Internal bar code: | GAGTTAGGCTCCAGCTATCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1247 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCTGGTCTACATACGCA |
| Suggested primer 2: | TAATCCCCATCCCTACTCCC |