Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.172607 |
Chromosome: | chromosome_11 |
Location: | 2737816 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g477100 | FAP97 | Flagellar Associated Protein, serine endopeptidase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AACACGGCCGCAACGGCACAATCCCCAGTG |
Internal bar code: | ATGTGGCCCTCGTGCCAAGAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 344 |
LEAP-Seq percent confirming: | 81.717 |
LEAP-Seq n confirming: | 3084 |
LEAP-Seq n nonconfirming: | 690 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCTCTGGTCTACATACGCA |
Suggested primer 2: | TAATCCCCATCCCTACTCCC |