Insertion junction: LMJ.RY0402.172607_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.172607
Chromosome:chromosome_11
Location:2737816
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre11.g477100 FAP97 Flagellar Associated Protein, serine endopeptidase antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):AACACGGCCGCAACGGCACAATCCCCAGTG
Internal bar code:ATGTGGCCCTCGTGCCAAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:344
LEAP-Seq percent confirming:81.717
LEAP-Seq n confirming:3084
LEAP-Seq n nonconfirming:690
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCTGGTCTACATACGCA
Suggested primer 2:TAATCCCCATCCCTACTCCC