Insertion junction: LMJ.RY0402.172658_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.172658
Chromosome:chromosome 11
Location:2104718
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g469224 (1 of 1) IPR010036//IPR023214 - Magnesium-dependent phosphatase-1, eukaryotic/arcaheal type // HAD-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCAGACGCTGGACCAGAACCTGCATGAA
Internal bar code:CACTTTGATGTCGTGGTGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:98.8007
LEAP-Seq n confirming:2389
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCGCAGTAGCGCTGTAG
Suggested primer 2:ATGCTTCCAGATGGAGTGCT