| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172658 |
| Chromosome: | chromosome 11 |
| Location: | 2104718 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g469224 | (1 of 1) IPR010036//IPR023214 - Magnesium-dependent phosphatase-1, eukaryotic/arcaheal type // HAD-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCGTGTTCACGGAAGCATTCTTTGAC |
| Internal bar code: | GGGATTTGATAGATGTCCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 959 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCGCAGTAGCGCTGTAG |
| Suggested primer 2: | ATGCTTCCAGATGGAGTGCT |