| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172677 |
| Chromosome: | chromosome 10 |
| Location: | 3055875 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441450 | (1 of 1) K14786 - protein KRI1 (KRI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACCTTTCCCCGCGCCTCACCCGCATC |
| Internal bar code: | ATTGGTGCGGATGACAAGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 99.7582 |
| LEAP-Seq n confirming: | 1650 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGTCGTCCACCCTGCATC |
| Suggested primer 2: | CCACCAACCGACACACATAC |