| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172680 |
| Chromosome: | chromosome 7 |
| Location: | 495709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315900 | Putative cargo transport protein EMP24; (1 of 1) PTHR22811:SF58 - TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN 10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACCTTGTGAGGACCTGGCACGTCGCCT |
| Internal bar code: | ATCCGTCCAGAGTGCCATTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.5212 |
| LEAP-Seq n confirming: | 6651 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTCTCCTTTTGCGGCCTA |
| Suggested primer 2: | TTCAAGCGGAAGAAGCTGAT |