| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172685 |
| Chromosome: | chromosome 12 |
| Location: | 9723076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557503 | (1 of 5) PF13676 - TIR domain (TIR_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACTGCATCGGTTAACCACACCCTGCAT |
| Internal bar code: | AAGCGCCTGTGTCTAAGAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 1053 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGATCTTAGTGTACCCC |
| Suggested primer 2: | CTTCCTGTGTGATGCTCGAA |