| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172693 |
| Chromosome: | chromosome 6 |
| Location: | 3059064 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274500 | (1 of 1) K14396 - polyadenylate-binding protein 2 (PABPN1, PABP2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCGGGTCCGTTGTCGCGGAGCGTGCCT |
| Internal bar code: | AGAATCGCGATGGTGTCGTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 99.8296 |
| LEAP-Seq n confirming: | 586 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGCAACTTTATGGGAGG |
| Suggested primer 2: | CAGCGAGCCTCATATGTTCA |