| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172729 |
| Chromosome: | chromosome 5 |
| Location: | 913615 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247300 | (1 of 1) PF13432//PF13489 - Tetratricopeptide repeat (TPR_16) // Methyltransferase domain (Methyltransf_23) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCCTTCATCTGCACCCGTGCCTGTGG |
| Internal bar code: | TTGCGGAGTCCCACTGGCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 76.0356 |
| LEAP-Seq n confirming: | 16759 |
| LEAP-Seq n nonconfirming: | 5282 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGGAAGACTGGAGGTG |
| Suggested primer 2: | TCTCACACTGGCTCTCATGG |