| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172765 |
| Chromosome: | chromosome 1 |
| Location: | 1543418 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008000 | (1 of 1) K18666 - activating signal cointegrator complex subunit 1 (ASCC1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAACATAACCCCCATAACCCTTCATATA |
| Internal bar code: | TTGGTTACGGCCGATCGGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 77.1429 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTTCACACACTCCAGGT |
| Suggested primer 2: | GCTCCGCTCGTTTTAACTTG |