| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172776 |
| Chromosome: | chromosome_1 |
| Location: | 7239066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g052150 | ANK2,FAP87 | Flagellar Associated Protein with ankyrin repeats | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGAGGTGGGCTCCATGGCTGGCTCACTTT |
| Internal bar code: | GGGCCTCCAGGATACACATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 81.6901 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTGTCATTGCGGTTGTT |
| Suggested primer 2: | GCACTCACTTTTGACCCCAT |