| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172776 |
| Chromosome: | chromosome 12 |
| Location: | 2458969 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506850 | (1 of 2) PTHR10183//PTHR10183:SF323 - CALPAIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGGGGGGTAACTTTGAACCAACCGTG |
| Internal bar code: | CTTCAGCTGGATTGATCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 34.8946 |
| LEAP-Seq n confirming: | 596 |
| LEAP-Seq n nonconfirming: | 1112 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCACCAAGCGAGAATGTG |
| Suggested primer 2: | TAGGTACCAGCCCAAACTGG |