| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172776 |
| Chromosome: | chromosome 16 |
| Location: | 1129403 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650000 | (1 of 19) PF01370 - NAD dependent epimerase/dehydratase family (Epimerase) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCTATTCATGTTGTGTCAGTTTTGCG |
| Internal bar code: | ACAGAAGCGATTTTGGGATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1147 |
| LEAP-Seq percent confirming: | 99.542 |
| LEAP-Seq n confirming: | 6303 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTATGTGTGCCCTGATGC |
| Suggested primer 2: | AACACCGATCACGCCTTTAC |