| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172779 |
| Chromosome: | chromosome 17 |
| Location: | 3401690 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724100 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGTCGTGGTGAGCCGCTCCGAAAGCCTC |
| Internal bar code: | TATAGTCGGGCCTGCAGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1135 |
| LEAP-Seq percent confirming: | 99.8277 |
| LEAP-Seq n confirming: | 1159 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATAATAATGCGTGGGTCC |
| Suggested primer 2: | GAGAGCATTACGGTGTGGGT |