| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172794 |
| Chromosome: | chromosome 13 |
| Location: | 2599076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581200 | (1 of 1) PTHR12241//PTHR12241:SF1 - TUBULIN TYROSINE LIGASE-RELATED // TUBULIN--TYROSINE LIGASE PBY1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGCTGAATACAGGCAGGGCTAGCCAGG |
| Internal bar code: | GGGTTTGGTACGCACGCCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 99.9301 |
| LEAP-Seq n confirming: | 1429 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCCCATCACACACCAAG |
| Suggested primer 2: | GACTCGTACCGCTTCACCTC |