| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172825 |
| Chromosome: | chromosome 7 |
| Location: | 454855 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315450 | CSG2 | Predicted protein of CSG family | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGCCCAACGCATCCGCCAATGCCAGCG |
| Internal bar code: | TATAGGGGCTCAATCCGGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 99.676 |
| LEAP-Seq n confirming: | 3076 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACGTGTAGCGGTGTAGT |
| Suggested primer 2: | TTACAGTCTGCATGCCTTCG |