| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172834 |
| Chromosome: | chromosome 9 |
| Location: | 7292068 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412750 | (1 of 1) K06228 - fused (FU) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGACCGACAACTGACTGACGAATGTCG |
| Internal bar code: | TTCGAACTGGATGTCGGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 99.2696 |
| LEAP-Seq n confirming: | 6388 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTTACACGCAGCTTCCC |
| Suggested primer 2: | CTCCCTCTCGTGCATCCTAC |