| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.172858 |
| Chromosome: | chromosome 6 |
| Location: | 3188721 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275750 | HTR4,HTR12 | Histone H3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGTTTTTTGGGGGGACAAACCAGCCCA |
| Internal bar code: | CTGCACTGTGTTCGTCGTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 53.8058 |
| LEAP-Seq n confirming: | 205 |
| LEAP-Seq n nonconfirming: | 176 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCTCTTGGGGACTTGTA |
| Suggested primer 2: | TCCTCGTAGATCAGACCGCT |