| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172877 |
| Chromosome: | chromosome 1 |
| Location: | 2353334 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012900 | (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTGCTCACAACACGACATGGCTTGTT |
| Internal bar code: | AGTCGAGTGGAAGTAAGGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1199 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATACGGTTACGCTGGAAT |
| Suggested primer 2: | TGTGCATGTCTCCAATGGTT |