| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.172899 |
| Chromosome: | chromosome 6 |
| Location: | 5739112 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287100 | CAM11 | Calmodulin-like protein; (1 of 2) PF13499//PF13833 - EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTCAGAGTTTACCCTCAACACACACGC |
| Internal bar code: | ACACGGGGTGGAACTTCGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 99.8565 |
| LEAP-Seq n confirming: | 1392 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTTTTCGGTTCGTCTC |
| Suggested primer 2: | CGCAGAGGACGGTAAAGAAG |