| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172922 |
| Chromosome: | chromosome 16 |
| Location: | 7682589 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690431 | (1 of 1) 1.3.99.34 - Transferred entry: 1.3.7.11 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCTCCGCCACCGGCACCGTTGTGCGCG |
| Internal bar code: | GGCAACTCATAGTAGACAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.5138 |
| LEAP-Seq n confirming: | 614 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGCATCAGAACGCGATA |
| Suggested primer 2: | CTAAGTTCGTCGAGAACGCC |