Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.172937 |
Chromosome: | chromosome 12 |
Location: | 2280766 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g507872 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCTCTGTTTGCTCTGCTGCCGTAAAGC |
Internal bar code: | GACGGGGGGCAGCACATGATCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 510 |
LEAP-Seq percent confirming: | 99.7904 |
LEAP-Seq n confirming: | 952 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAATTGTTTGCTGGGGTCTG |
Suggested primer 2: | GTAGGGTGGGGTGAGACAGA |