Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.172958 |
Chromosome: | chromosome_8 |
Location: | 1127939 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362650 | IFT140 | Intraflagellar transport protein 140 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATTATCAACTTGCACTTCACCTTACCCTC |
Internal bar code: | TCGCCACACGTCGCATGGCAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 963 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 1406 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGACACAGGGTTCAAATGCT |
Suggested primer 2: | AACACAACCAAACTCCAGGC |