Insertion junction: LMJ.RY0402.172967_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.172967
Chromosome:chromosome 3
Location:6200229
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g192350 (1 of 2) IPR006652//IPR015915 - Kelch repeat type 1 // Kelch-type beta propeller 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCCAGAGTTGAGCGCAGTCCCGCGACCG
Internal bar code:TGGTGACGGATTACCCCCCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:160
LEAP-Seq percent confirming:98.5869
LEAP-Seq n confirming:1186
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGAGCATGCTTCGGAACT
Suggested primer 2:CCCTGCTCTACCTACCCTCC