| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.172971 |
| Chromosome: | chromosome 13 |
| Location: | 2441847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579800 | (1 of 1) 2.7.4.26 - Isopentenyl phosphate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCTGTGAGGCTGTGACGCCATGGGCCA |
| Internal bar code: | TTAAACATTTGGCTATCGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 99.3205 |
| LEAP-Seq n confirming: | 1754 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCATCCTTTTCAAGCTC |
| Suggested primer 2: | ACTAAACCAGATGCGGTTGG |