| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.172992 |
| Chromosome: | chromosome 16 |
| Location: | 3874433 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690100 | FAP213 | Coiled-Coil Flagellar Associated Protein 213 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCGGGCGAGATGAACGCCAAGAAGCAG |
| Internal bar code: | CGGGCATTCTTAGACTTCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 85.6742 |
| LEAP-Seq n confirming: | 610 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAGATGAGAGTGTCGGA |
| Suggested primer 2: | CTGGTGTAGGACCCGACATT |