Insertion junction: LMJ.RY0402.173002_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.173002
Chromosome:chromosome 4
Location:3734191
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g229350 (1 of 1) 2.1.1.290 - tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase / tRNA-yW synthesizing enzyme-4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGAGACTTTACCTTGACGCACATTCCAA
Internal bar code:TGCGCCGGCGGTTGGTGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:507
LEAP-Seq percent confirming:99.3631
LEAP-Seq n confirming:156
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGACTCTAGTGGCTGCAT
Suggested primer 2:CTCACTCAGCACCCACAAAA