| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173038 |
| Chromosome: | chromosome 1 |
| Location: | 6340062 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045902 | HCF101 | [4Fe-4S]-cluster scaffold protein; (1 of 1) PTHR23264//PTHR23264:SF29 - NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED // PROTEIN HIGH CHLOROPHYLL FLUORESCENCE 101 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGAACTATTTCGTTGCAGTCGGGCAG |
| Internal bar code: | GCATGTGCGGCACTTGGGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 42 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTGTATGTTGGGAGGAC |
| Suggested primer 2: | CCACCTGACGTCGGAGTTAT |