| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173094 |
| Chromosome: | chromosome 10 |
| Location: | 542763 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421350 | MMP25 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGTGGGCTGTCACGCCGCGCCTGCGG |
| Internal bar code: | CCGGTTCTTTGGGCCTGTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 904 |
| LEAP-Seq percent confirming: | 99.4574 |
| LEAP-Seq n confirming: | 9165 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGGCTCAAGACACATC |
| Suggested primer 2: | TGCTAGAATGAAGGCGTGTG |