| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173105 |
| Chromosome: | chromosome_4 |
| Location: | 2934155 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g224650 | FAP296 | Flagellar Associated importin alpha-like protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCAGTACGGGGCTGCGTGCCACACCCAC |
| Internal bar code: | CCCGCGACCTATGCCTTACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 89.3293 |
| LEAP-Seq n confirming: | 8045 |
| LEAP-Seq n nonconfirming: | 961 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCTATCAGCGCTTCATT |
| Suggested primer 2: | AGTGAGGTTAGAGCCAGGCA |