Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.173105 |
Chromosome: | chromosome_5 |
Location: | 1272971 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
None |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAAAGTTATGGTGGCGAAGCCACAGCCTAG |
Internal bar code: | CGAAACGATCGCCACACAACTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 375 |
LEAP-Seq percent confirming: | 99.5248 |
LEAP-Seq n confirming: | 1466 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGGACGCAGCAAACTATGA |
Suggested primer 2: | TCACGGCCTTTCCTTATGTC |