| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173112 |
| Chromosome: | chromosome 9 |
| Location: | 3127560 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386912 | DGTT2 | Diacylglycerol acyltransferase, DGAT Type 2; (1 of 4) K14457 - 2-acylglycerol O-acyltransferase 2 (MOGAT2, MGAT2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTTAGGCCATCTGTTTTTGATTAAAATT |
| Internal bar code: | CGCCACGGGTGAAGCGAAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1036 |
| LEAP-Seq percent confirming: | 98.0695 |
| LEAP-Seq n confirming: | 3556 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCTTCCATCACACACAC |
| Suggested primer 2: | ACCCGATCCACACAAGTAGC |