| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173128 |
| Chromosome: | chromosome 17 |
| Location: | 4666036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g733400 | (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCTTTGTTGTAGACATAGCTGGGGGGC |
| Internal bar code: | CGATTTGGCCGGTTCCCTGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 98.5916 |
| LEAP-Seq n confirming: | 700 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCATGTTCGCGCTTAT |
| Suggested primer 2: | TGTCAAAATTCGCGCTGTAG |