| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173181 |
| Chromosome: | chromosome 16 |
| Location: | 4774756 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686550 | (1 of 1) PF12400 - Vaculolar membrane protein (DUF3661) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCCACCACTCCTGCATCCATGCACCC |
| Internal bar code: | ATAACCTCGTGGGACCAAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 82.5444 |
| LEAP-Seq n confirming: | 837 |
| LEAP-Seq n nonconfirming: | 177 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTATGTACGCATCAGGTG |
| Suggested primer 2: | CGGAGGAGAGATTAAAGGGG |