Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.173222 |
Chromosome: | chromosome_9 |
Location: | 3235677 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g387689 | FAP76 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAATGAGGATGTCATTGCGGCATCTGTCAC |
Internal bar code: | ACCGCTGAGCGCCCTCGCATGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 678 |
LEAP-Seq percent confirming: | 99.6121 |
LEAP-Seq n confirming: | 1284 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTCGCCTAGCCACCTAGT |
Suggested primer 2: | CTGTGTGCTACTGACCGCAT |