| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173227 |
| Chromosome: | chromosome 12 |
| Location: | 9164542 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542300 | GLYK,GYK1,GLYK1 | Glycerate kinase; (1 of 1) 2.7.1.31 - Glycerate 3-kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAAGCTCATGCCCGTGTTGTGGAGGC |
| Internal bar code: | AGTTGACGCCCGTGGCTAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 99.5101 |
| LEAP-Seq n confirming: | 1422 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCTCCCCTCAGACCATC |
| Suggested primer 2: | GATTCTTCGCACAACAGCAA |