Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.173243 |
Chromosome: | chromosome 6 |
Location: | 5960219 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g288908 | (1 of 30) PTHR24349//PTHR24349:SF79 - SERINE/THREONINE-PROTEIN KINASE // NITROGEN NETWORK KINASE 1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCAAAACGGGTTGACGGGTATCTACTT |
Internal bar code: | CCCCACGAGTTGGGACGTTAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 624 |
LEAP-Seq percent confirming: | 99.8295 |
LEAP-Seq n confirming: | 1171 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCCAACCTGACTCCTCACC |
Suggested primer 2: | TCTACCACGTGCTCAAGGTG |