| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173269 |
| Chromosome: | chromosome 6 |
| Location: | 8187781 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305350 | (1 of 1) PTHR11319//PTHR11319:SF32 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAAGAAGTTATAGCGTCCGCACGCCGTC |
| Internal bar code: | ATTGCGTTTTCAGGGGTTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 917 |
| LEAP-Seq percent confirming: | 99.4543 |
| LEAP-Seq n confirming: | 2187 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTTCAATCTTTGCGCTC |
| Suggested primer 2: | AGTGCTATGCCCGTGGTATC |