| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173270 |
| Chromosome: | chromosome 12 |
| Location: | 7991967 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552001 | (1 of 4) PF01435 - Peptidase family M48 (Peptidase_M48) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTGTTGTTGCTGTTGGGGAGTTGTGGA |
| Internal bar code: | AGCGGGAGTGATGTCCCCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 99.7176 |
| LEAP-Seq n confirming: | 6709 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCTACGTCAAATGCCGC |
| Suggested primer 2: | ATTGGCAAGAATTTACCCCC |