Insertion junction: LMJ.RY0402.173300_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.173300
Chromosome:chromosome 9
Location:4111857
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g393654 RLS11 RegA/Rls-like protein; (1 of 10) IPR010919 - SAND domain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTAGTGCTGCGCCCGAGCATGATGGCAT
Internal bar code:GTCAACATGGAGACTGATATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:43.6314
LEAP-Seq n confirming:322
LEAP-Seq n nonconfirming:416
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCAATAGACGCATGTCTCGC
Suggested primer 2:CCGAAGAAACCTAGCTGACG