Insertion junction: LMJ.RY0402.173354_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.173354
Chromosome:chromosome 7
Location:4555316
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g343850 (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGCTACGCCTTCACTTCTTAAAACATG
Internal bar code:CACTTGGATTCTCCTGTCGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:20
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTGGAAGTTGGTGGAG
Suggested primer 2:GACCAGCCCAAACTAAACCA